Figure 2. Rescue of growth and developmental defects in the csn-5^KO strain by the expression of JAMM-motif mutant CSN-5.

(A) Wild-type, csn-5^KO, and the different CSN-5 complementation strains growing on slants containing QA. csn-5^KO strains produced significantly less conidia and aerial hyphae than wild-type or CSN-5 complementation strains. (B) Growth rates of the wild-type, csn-5^KO, and CSN-5 complementation strains, measured at 25°C by race tube assays in constant darkness after 1 d of light treatment. (C–E) Rescue of conidiation rhythms in the different CSN-5 complementation strains, measured by race tube assay in dark–dark (C), light–dark (D), and temperature cycles (E). At least four replicates were tested under each condition. Black lines indicate the growth fronts every 24 h.