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. 2012 May 10;8(5):e1002683. doi: 10.1371/journal.ppat.1002683

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Gutiérrez-Escribano et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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BCR1 phosphomimetic (bcr1 ^T191E/bcr1Δ, JC1094; bcr1 ^S556E/bcr1Δ, JC1092; bcr1EE/bcr1Δ, JC1180) and phosphodefective (bcr1 ^T191A/bcr1Δ, JC1093; bcr1 ^S556A/bcr1Δ, JC1088; bcr1AA/bcr1Δ, JC1178) mutants were grown in biofilm-inducing conditions for 40 hours using microfermentors. BCR1/bcr1Δ (WT, JC1089) and bcr1Δ (JC1081) strains were used as controls. (A) Quantification of adherent cells. (B) Biofilm dry mass determination. (C) Biofilms grown using silicon squares models were stained with calcofluor white for CSLM visualization.