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. 2012 May 10;8(5):e1002683. doi: 10.1371/journal.ppat.1002683

Figure 5. Characterization of the in vitro biofilm formation phenotype of the cbk1Δ bcr1EE double mutant.

Figure 5

(A) Quantification of adherence ability to plastic slides of wild-type (JC1089), cbk1Δ BCR1 (JC1082), cbk1Δ bcr1AA (JC1096) and cbk1Δ bcr1EE (JC1099) cells. (B) The same strains were incubated during 40 hours in microfermentors and biofilm dry mass was determined. (C) CSLM images of biofilms induced using silicone squares after calcofluor white staining. (D) Quantitative RT-PCR measurements of ALS3 and ALS1 transcription levels in the wild-type, cbk1Δ BCR1 and cbk1Δ bcr1EE strains normalized using ADE2.