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. 2001 May 15;98(11):6506–6510. doi: 10.1073/pnas.111423098

Figure 2.

Figure 2

CMV and PVY, but not PVX, suppress PTGS in TSC. RNA blot hybridization with a CHN cDNA probe and immunoblot analyses of leaves from sibling STSC plants (STSC1–STSC4). Tissues were harvested from the inoculated leaf just before inoculation (0), from leaves showing systemic virus infection 10–15 DPI (V), and from leaves showing systemic virus infection 2 days later after treatment of plants with 20-ppm ethylene (+ET). Total RNA (10 μg) was hybridized with a CHN48 cDNA probe, which detects CHN48 and CHN50 RNA (CHN RNA). The double bands detected with the probe have been described earlier and may result from alternative polyadenylation (26). Equal loading was confirmed by rehybridization with a probe for 18S rRNA. Immunoblot analyses of equal volumes of protein extracts of the same tissues used for RNA analyses are shown at the bottom. The positions of the class I chitinases CHN48, its truncated form ΔCHN48 and CHN50, and the class II chitinases (CHN II) are indicated.