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. 2001 May 15;98(11):6506–6510. doi: 10.1073/pnas.111423098

Figure 3.

Figure 3

CMV and PVY, but not PVX, suppress accumulation of transgene-encoded antisense transcripts in TAC and TAG. Tissues were harvested from the inoculated leaf just before inoculation (0) and from leaves showing systemic virus infection 10–15 DPI of sibling TAC (TAC1–TAC4) and TAG (TAG1–TAG4) plants. RNA blot hybridization of total RNA (10 μg) by using RNA probes to detect antisense CHN RNA (A) or antisense GLU RNA (B). Equal loading was confirmed by rehybridization with a probe for 18S rRNA.