Skip to main content
. 2001 May 15;98(11):6506–6510. doi: 10.1073/pnas.111423098

Figure 4.

Figure 4

CMV and PVY, but not PVX, restore ethylene-induction of endogenous-gene expression in antisense transformants. RNA blot and immunoblot analyses of leaf tissues harvested as indicated in Fig. 2 from sibling TAC plants (A) and TAG (B) plants. Total RNA (10 μg) was hybridized with RNA probes for detecting sense CHN RNA (A) or sense GLU RNA (B). Equal loading was confirmed by rehybridization with a probe for 18S rRNA. Protein extracts representing equal amounts of the same tissues were immunoblotted by using probes for CHN antigens and GLU antigens indicated in Fig. 2. Purified GLU I protein was used as size marker (B, GLU I). Positions of GLU I and the class II (GLU II) and class III (GLU III) β-1,3-glucanases are indicated. Note that induction of GLU I antigen is suppressed in mock- and PVX-infected plants, but not in CMV- and PVY-infected plants.