Figure 5.

Small, ca. 25-nt sense- and antisense-RNAs are associated with both PTGS and ASGS. RNA blot hybridization of 20 μg of the low-molecular-weight RNA fraction prepared from total RNA of untreated plants. Membranes were hybridized with RNA probes for sense and antisense CHN (A) and GLU (B) RNA. The position of the ca. 25-nt RNAs was determined by using single-stranded DNA primers as size markers. Unspecific cross-hybridization with tRNAs (top of each gel) was used as the control for equal loading. Note that an additional ca. 30-nt RNA was detected with sense and antisense GLU probes. This RNA was present at roughly the same abundance in wild type and transformed lines and was not correlated with ASGS.