Fig. 5.

SAAS-derived matrix describing 8-mer binding to Mamu-B*052:01. A panel of single amino acid substitution analogs of the Mamu-B*052:01 binding 7.4 (sequence VGNVYVKF) was tested for binding, and the data analyzed, as described for the PSCL in the “Materials and methods”. Because of the larger SD in the panels of SAAS, compared to PSCL, more stringent criteria to define primary and secondary anchor positions have been utilized, as also described in the “Materials and methods”. At the primary anchor positions (SF > 3.5; blue shading), the most preferred residues, associated with an ARB > 0.1 are highlighted by bold yellow font. Green shading highlights secondary anchor positions. The library average binding for Mamu-B*052:01 was 18.0 nM