Figure 2.

Claudin 3 gene expression peaks at 3 weeks in the murine intestine. Quantitative real-time RT-PCR of TJ protein genes undergoing developmental changes in expression in murine intestines collected during the first 8 weeks of life. A: Developmental survey of TJ protein gene expression. Bars represent fold induction when comparing mRNA levels in 3-week-old versus 2-day-old mice. B and C: mRNA expression of claudin 3 (B) and claudin 7 (C) over time. Data are expressed as fold change (mean ± SEM from at least three independent experiments) versus 2 days old (set to 1).