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. 2012 Feb;180(2):626–635. doi: 10.1016/j.ajpath.2011.10.025

Figure 4.

Figure 4

Maturation of intestinal claudin 3 expression and barrier function is MyD88 dependent. A: Immunofluorescent localization of claudin 3 in wild-type (control), MyD88−/−, or antibiotic-raised (Abx) 3-week-old mice. Top panels: claudin 3 staining alone (green). Bottom panels: Nuclear counterstain (blue). B: Expression of CLDN3 and occludin (OCLN) protein by immunoblot in murine intestines at the age of 3 weeks. C: Relative intestinal mRNA expression of claudin 3 in wild-type (control), MyD88−/−, or antibiotic-raised 3-week-old mice, as assayed by quantitative real-time RT-PCR. Data are the mean ± SEM from at least three experimental repeats per condition. D: Intestinal permeability, as measured by serum FD4 concentration 5 hours after oral gavage in wild-type (control), MyD88−/−, or antibiotic-raised 3-week-old mice. Data are the mean ± SEM from at least three experimental repeats per condition. *P < 0.05 versus control.