Figure 6.
LGG induces claudin 3 expression. A: Relative intestinal mRNA expression of claudin 3, as assayed by quantitative real-time RT-PCR in mice gavage fed PBS alone (control) or with LGG for 1 week. Intestines were harvested at the age of 2 weeks. Data are the mean ± SEM from at least three experimental repeats per condition. *P < 0.05 versus control. B: Immunofluorescent localization of claudin 3 in mice treated with PBS alone (control) or with LGG (1 × 109 CFU) for 1 week. Top panels: claudin staining alone (green). Bottom panels: Nuclear counterstain (blue).