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. 2012 Feb;180(2):738–748. doi: 10.1016/j.ajpath.2011.10.033

Figure 1.

Figure 1

Expression of DOX-inducible COMP in vitro. A: Schematic of the construct used to express WT-COMP and D469del-COMP. The ubiquitous cytomegalovirus promoter (pCMV) drives expression of rtTA, which in turn drives expression of the COMP gene in the presence of DOX. B: Western blots show WT-COMP and D469del-COMP expression. RCS cells were cultured in the presence of 1 μg/mL DOX for 2, 4, and 7 days. β-Actin was used as the loading control. C and D: Coimmunostaining of infected RCS cells with FLAG (green) and Golgi-specific 58K (red) at 4 days (C) or ER-specific CRT antibodies (red) at 7 days (D). DAPI (blue) indicates the nucleus. Scale bar = 5 μm.