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. 2012 Feb;180(2):738–748. doi: 10.1016/j.ajpath.2011.10.033

Figure 5.

Figure 5

Intracellular D469del-COMP induces DNA damage in RCS cells. mRNA levels of Gadd34 (A) and Gadd45a (B) were analyzed by qRT-PCR after the WT-COMP and D469del-COMP expression (dark bars) and retention (light bars) periods. Measurements were normalized to GAPDH. Assays were performed in triplicate; error bars indicate ± SD. *P < 0.05; **P < 0.01. C: Levels of γH2AX in RCS cells after the WT-COMP and D469del-COMP expression and retention periods were analyzed by Western blot. γH2AX expression was compared between negative controls, one with no virus (NV) and the other with rtTA virus (TA), and WT-COMP and D469del (MT)-COMP expressing viruses. β-Actin was used as the loading control.