Table 1.
Gene⁎ | Primer sequences |
---|---|
hCOMP | Fwd-5′-GCAATGACACCATCCCAGAG-3′ |
Rev-5′-CTTGTCATCGTCGTCCTTGTAGTC-3′ | |
Chop | Fwd-5′-CCAGCAGAGGTCACAAGCAC-3′ |
Rev-5′-CGCACTGACCACTCTGTTTC-3′ | |
Grp78 | Fwd-5′-CCACCAGGATGCAGACATTG-3′ |
Rev-5′-AGGGCCTCCACTTCCATAGA-3′ | |
Xbp1(S)† | Fwd-5′-GTCTGCTGAGTCCGCAGCAGGT-3′ |
Rev-5′-CAGACTCAGAATCTGAAGAGGC-3′ | |
Edem1 | Fwd-5′-GCAATGAAGGAGAAGGAGAC-3′ |
Rev-5′-CCATATGGCGTAGTAGAAGGC-3′ | |
Gadd34 | Fwd-5′-AATCAGGACCCTGAGATTCCT-3′ |
Rev-5′-CTGGTCCTGCCCAGACAG-3′ | |
Ero1b | Fwd-5′-GACACCAAGACCCTTCTGCT-3′ |
Rev-5′-CTTTAATGACTTGGCCCCC-3′ | |
Gadd45a | Fwd-5′-CCGAAAGGATGGACACGGTG-3′ |
Rev-5′-TTATCGGGGTCTACGTTGAGC-3′ | |
Gapdh | Fwd-5′-AGTTCAACGGCACAGTCAAG-3′ |
Rev-5′-TACTCAGCACCAGCATCACC-3′ |
Alternative gene names and symbols include the following. For Chop: DNA damage-inducible transcript 3 protein (Ddit3). For Gadd34: protein phosphatase 1, regulatory subunit 15A (Ppp1r15a). For Grp78: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (Hspa5).
Spliced isoform.