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. 2012 Feb;180(2):738–748. doi: 10.1016/j.ajpath.2011.10.033

Table 1.

Primers Used in Real-Time RT-PCR

Gene Primer sequences
hCOMP Fwd-5′-GCAATGACACCATCCCAGAG-3′
Rev-5′-CTTGTCATCGTCGTCCTTGTAGTC-3′
Chop Fwd-5′-CCAGCAGAGGTCACAAGCAC-3′
Rev-5′-CGCACTGACCACTCTGTTTC-3′
Grp78 Fwd-5′-CCACCAGGATGCAGACATTG-3′
Rev-5′-AGGGCCTCCACTTCCATAGA-3′
Xbp1(S) Fwd-5′-GTCTGCTGAGTCCGCAGCAGGT-3′
Rev-5′-CAGACTCAGAATCTGAAGAGGC-3′
Edem1 Fwd-5′-GCAATGAAGGAGAAGGAGAC-3′
Rev-5′-CCATATGGCGTAGTAGAAGGC-3′
Gadd34 Fwd-5′-AATCAGGACCCTGAGATTCCT-3′
Rev-5′-CTGGTCCTGCCCAGACAG-3′
Ero1b Fwd-5′-GACACCAAGACCCTTCTGCT-3′
Rev-5′-CTTTAATGACTTGGCCCCC-3′
Gadd45a Fwd-5′-CCGAAAGGATGGACACGGTG-3′
Rev-5′-TTATCGGGGTCTACGTTGAGC-3′
Gapdh Fwd-5′-AGTTCAACGGCACAGTCAAG-3′
Rev-5′-TACTCAGCACCAGCATCACC-3′

Alternative gene names and symbols include the following. For Chop: DNA damage-inducible transcript 3 protein (Ddit3). For Gadd34: protein phosphatase 1, regulatory subunit 15A (Ppp1r15a). For Grp78: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) (Hspa5).

Spliced isoform.