Table 2.
Genes Involved in Oxidative Stress That Are Regulated by Retention of MT-COMP in RCS Cells Compared with Control rtTA
| Rat gene symbol | Gene name | Fold change | P value |
|---|---|---|---|
| Antioxidants | |||
| Apc | Adenomatous polyposis coli | 11.32 | 0.0018 |
| Gsr | Glutathione reductase | 2.35 | 0.0065 |
| Gstk1 | Glutathione-S-transferase kappa1 | 4.42 | 0.032 |
| Prdx3 | Peroxiredoxin 3 | 6.15 | 0.0057 |
| Prdx5 | Peroxiredoxin 5 | 3.48 | 0.001 |
| Prdx6 | Peroxiredoxin 6 | 3.53 | 0.009 |
| Ptgs1 | Prostaglandin-endoperoxide synthase 1 | 2.08 | 0.01 |
| Ptgs2 | Prostaglandin-endoperoxide synthase 2 | 16.56 | 0.037 |
| Tmod1 | Tropomodulin 1 | 12.29 | 0.0031 |
| Txnrd1 | Thioredoxin reductase 1 | 5.36 | 0.0001 |
| Txnrd2 | Thioredoxin reductase 2 | 8.36 | 0.001 |
| Genes involved in reactive oxygen species metabolism | |||
| Genes involved in superoxide metabolism | |||
| Nox4 | NADPH oxidase 4 | 107.55 | 0.0030 |
| Oxidative stress responsive genes | |||
| Als2 | Amyotrophic lateral sclerosis (juvenile) homolog human | 4.53 | 0.0005 |
| Apoe | Apolipoprotein E | 3.73 | 0.0001 |
| Duox2 | Dual oxidase 2 | −2.77 | 0.037 |
| Ercc2 | Excision repair cross-complementing rodent repair deficiency, complementation group 2 | 5.09 | 0.0235 |
| Nqo1 | NAD(P)H dehydrogenase, quinone 1 | 4.25 | <0.0001 |
| Park7 | Parkinson disease (autosomal recessive, early onset) 7 | 2.34 | 0.0145 |
| Prnp | Prion protein | 10.58 | 0.0006 |
| Psmb5 | Proteasome (prosome, macropain) subunit, beta type 5 | 2.26 | 0.0001 |
| Txnip | Thioredoxin-interacting protein | 29.89 | 0.0016 |
| Oxygen transporter genes | |||
| Cygb | Cytoglobin | 4.85 | 0.0031 |
| Dnm2 | Dynamin 2 | 4.17 | 0.0402 |
| Fancc | Fanconi anemia, complementation group C | 3.92 | 0.0046 |
| Hba-a2 | Hemoglobin alpha, adult chain 2 | 8.85 | 0.0002 |
| Ift172 | Intraflagellar transport 172 homolog (Chlamydomonas) | 3.73 | 0.0025 |
| Slc38a1 | Solute carrier family 38, member 1 | 19.22 | 0.0076 |
| Slc38a4 | Solute carrier family 38, member 4 | 98.46 | 0.0028 |
Out of 84 genes present in the rat oxidative stress and antioxidant defense PCR array (PARN-065E RT2 Profiler PCR array, Qiagen SABiosciences, Frederick, MD), only genes that presented a twofold or greater change with significance at P < 0.05 are shown.