Table 1.
Tissues Used for Cell Isolation and Culture
| Species | Tissue type | Cases | Total samples | Cell lines | Normal/diseased |
|---|---|---|---|---|---|
| Human | Breast | 13 | 25 | 25 | 18/4B/3T |
| Prostate | 9 | 16 | 16 | 9/7T | |
| Lung | 6 | 8 | 8 | 5/3T | |
| Liver | 5 | 6 | 3 | 1/2T | |
| Colon | 4 | 8 | 4 | 1/4T | |
| Pancreas | 5 | 8 | 4 | 2/2T | |
| Endothelium | 1 | 1 | 1 | 1/0 | |
| Ovary | 1 | 1 | 1 | 1/0 | |
| Pancreas (met) | 1 | 1 | 1 | 0/1T | |
| Oral (H&N) | 2 | 3 | 3 | 1/2T | |
| Mouse | Breast | 7 | 7 | 7 | 6/1T |
| Prostate | 3 | 3 | 3 | 3/0 | |
| Rat | Breast | 5 | 5 | 5 | 1/4T |
| Dog | Skin epithelium | 1 | 1 | 1 | 1/0 |
| Oral epithelium | 1 | 1 | 1 | 1/0 | |
| Total | NA | 64 | 94 | 83 | NA |
The tissues for which biopsy specimens were obtained for culture are indicated for each of the mammalian species. The number of cases (patient or animal) is indicated, as is the total number of samples taken from those cases. The cell lines that were generated from these samples are also indicated, and the success rate was determined by dividing the number of generated cell lines by the total number of samples placed into tissue culture. The success rate is defined as the ability of the cultured cells to reach 50 population doublings or, in some cases (colon and pancreas), as three times the population doublings observed with current, optimal culture conditions.
B, BRCA1/2 mutation carrier; H&N, head and neck cancer; met, metastatic; NA, not applicable; T, tumor.