Figure 5.

CD103⁺ LP DCs from CRBPII^−/− mice have an impaired ability to facilitate IgA production. To assess for a defect in CD103⁺ LP DCs from CRBPII^−/− mice related to their reduced ALDH activity, CD103⁺ and CD103⁻ LP DCs from CRBPII^−/− mice and wild-type mice were isolated and cultured with naive B cells and evaluated for the development of IgA-producing plasma cells by enzyme-linked immunosorbent spot (A) and IgA production by ELISA (B) as described in Materials and Methods. CD103⁺ LP DCs from wild-type mice had an augmented capacity to facilitate IgA production, whereas CD103⁺ LP DCs from CRBPII^−/− mice were less effective at facilitating IgA production and were comparable to the CD103⁻ population. *P < 0.05. Data are representative of one of two experiments.