Figure 2.

p47^phox protein attenuates IL-4 signaling in macrophages independent of the gp91^phox catalytic subunit. A: Immunoblot analysis of STAT6 phosphorylation in inflammatory macrophages from each genotype stimulated with IL-4 for 20 minutes as indicated. Results of one representative experiment of three independent experiments are shown. B: Real-time PCR analysis of Arg1, Ym, and FIZZ1 mRNA from inflammatory macrophages from each genotype stimulated with IL-4 for 18 hours as indicated. Values are relative to expression of the gene encoding RPS29. Data represent three independent experiments including three of each genotype per experiment. *P < 0.05.