Figure 1.

Plexin-B1 and Sema4D are highly expressed in neurotropic tumor and nerve cell lines but not in normal or nonneurotropic controls. A: SDS-PAGE immunoblot for plexin-B1 and Sema4D in the HNSCC lines HN12 and HN13 and the corresponding nontransformed control line, immortalized normal oral keratinocytes (INOKs), and the pancreatic cancer cell lines, HPAC and Capan-1, compared with nontransformed control HPNE cells. B: Du-145 and PC3, prostate cancer cell lines that exhibit PNI, express high levels of plexin-B1 and Sema4D compared with the nontransformed controls HPR-1 and BPH-1 (from benign prostatic hypertrophy), or LnCAP cells, which exhibit low PNI in xenografts. C: Plexin-B1 and Sema4D expression in the nerve cell lines HCN-1A and sNF96.2. GAPDH was used as a loading control for all immunoblots.