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. 2012 Mar;180(3):1232–1242. doi: 10.1016/j.ajpath.2011.12.009

Figure 4.

Figure 4

Tumor cells expressing plexin-B1 are attracted to nerves in vivo. A: Tumors derived from PC3 cells infected with a control lentivirus (C) and tumors from plexin-B1 shRNA-infected cells are shown in hematoxylin-stained sections. More PNI is seen in tumors derived from control PC3 cells compared with tumors derived from plexin-B1 shRNA-infected cells. The number of nerves exhibiting PNI per total nerves counted in xenografts derived from control and plexin-B1 shRNA-infected PC3 cells is shown in the bar graph. *P < 0.05. B: Tumors derived from LnCAP cells infected with a control lentivirus (C) or a virus expressing wild-type plexin-B1 are shown in hematoxylin-stained sections. More PNI is seen in tumors derived from cells overexpressing plexin-B1 compared with controls. The number of nerves exhibiting PNI per total nerves counted in xenografts derived from control and plexin-B1–infected LnCAP are shown in the bar graph. *P < 0.05. Nerves are highlighted by neurofilament staining in IHC analysis of tumor tissues. Original magnification, ×40 (all panels).