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. 2001 May 1;98(11):6511–6515. doi: 10.1073/pnas.091114198

Figure 1.

Figure 1

Schematic genetic and physical representation of L. esculentum linkage group (A) bordering the Ve gene. Analysis of populations segregating for Ve identified closely linked codominant random-amplified polymorphic DNAs and allele-specific SCARs that map to the region of RFLP GP39. Identification of contiguous λ-genomic clones (B) facilitated the subcloning of genomic DNA (C) containing the Ve locus. Vertical lines indicate the location of the AUG initiation codon in the subgenomic clones. Expressed sequences were cloned into λ, and arrowheads depict the direction of transcription for the cDNAs (D) identified by using the genomic clone pG1Ve. Potato plants transformed with the genomic subclones pG2Ve and pG3Ve or cDNAs pC1Ve and pC2Ve exhibited in vivo complementation and resistance (R) when challenged with V. albo-atrum race 1. Potato plants transformed with the genomic pG1Ve and the binary vectors pBIN19 and pBI121 were susceptible (S) to infection.