Table 1.
Disease incidence in potato and tomato genomes challenged with aggressive isolates of V. albo-atrum race 1 and P. infestans A2 US8
| Line* |
V. albo-atrum race
1
|
P. infestans A2 US8
|
||
|---|---|---|---|---|
| R | S | R | S | |
| Potato | ||||
| pG1Ve | 0 | 31 | 0 | 8 |
| pG2Ve | 48 | 0 | 0 | 8 |
| pG3Ve | 83 | 0 | 0 | 8 |
| pC1Ve | 30 | 0 | 0 | 8 |
| pC2Ve | 78 | 0 | 0 | 8 |
| pBIN | 0 | 48 | 0 | 8 |
| pBI122 | 0 | 48 | 0 | 8 |
| Désirée | 0 | 56 | 0 | 8 |
| Tomato | ||||
| Ailsa Craig | 0 | 32 | 0 | 32 |
| Craigella | 32 | 0 | 0 | 32 |
Plants were rated 3 weeks postinoculation as resistant (R, no disease symptoms) or susceptible (S, advanced necrosis). Complementation was observed only in plants challenged with V. albo-atrum race 1 expressing full-length genomic DNA or cDNA of Ve1 (pG2Ve and pC1Ve) or Ve2 (pG3Ve and pC2Ve).
Transformants pG1Ve, pG2Ve, and pG3Ve contain genomic DNA with the intergenic region and N terminus of Ve1 and Ve2, a full-length clone of Ve1, or a full-length clone of Ve2, respectively. Plants transformed with pC1Ve (Ve1) and pC2Ve (Ve2) express full-length cDNA clones under the transcriptional control of the cauliflower mosaic virus 35S promoter.