Effect of AIB1/SRC-3 on the inflammatory response and MMP activity during wound healing. A: CD14 and CD16 mRNA expression in wounds and bone marrow on days 0, 1, and 4 after injury. Values are given as the mean ± SEM (n = 3 animals per time point and genotype). *P < 0.001, **P < 0.0001. B: Hematopoietic, mesenchymal stem cell, and proinflammatory marker mRNA expression in bone marrow of AIB1/SRC-3+/+ and +/− mice. Values are given as the mean ± SEM (n = 3 mice per group). C: Staining of granulation tissue of day 5 wounds for F4/80-positive macrophages (arrowheads). Scale bars: 0.1 mm. D: Number of F4/80-positive macrophages invading the granulation in five nonoverlapping visual fields. Values are given as the mean ± SEM (n = 3 to 4 animals per group). *P < 0.001 versus control. E: Representative images of fluorescent activatable MMP substrate in day 3 wounds. Four wounds per animal, with an approximate mean diameter of 3 mm, are visible (also see Figure 3A). The color represents signal intensity ranges from red (highest activity) to blue (lowest activity). F: Quantitation of MMP activity. Values are given as the mean ± SEM (n = 4 animals per group). ***P < 0.05 versus control. Details and a full time course of MMP activity are given in Supplemental Figure S8 (available at http://ajp.amjpathol.org).