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. Author manuscript; available in PMC: 2013 May 7.
Published in final edited form as: Mol Pharm. 2012 Mar 28;9(5):1361–1373. doi: 10.1021/mp200623w

Figure 5.

Figure 5

SDS-PAGE analysis of expression and purification of four CPP-dmCocE fusion proteins (a) Tat-N-dmCocE, (b) LMWP-N-dmCocE, (c) dmCocE-C-Tat, and (d) dmCocE-C-LMWP. Lane M: Mark12™ protein molecular weight standard (Invitrogen); Lane P: the pellet fraction of cell lysate; Lane L: the supernatant fraction of cell lysate; Lane FT: flow-through fraction; Lane W: wash fraction; Lane 1–5: elution fraction containing dmCocE. Lane FT to Lane 5 were obtained after passing through from the Talon™ column.