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Reaction Mixtures for Assay of Galactosyltransferase Activity
| Sample | Dilution | Vol. (μl) of diluted sample | Vol. (μl) of 200 mM GlcNAc | Vol. (μl) 10× Gal labeling buffer | Vol. (μl) Milli-Q water |
|---|---|---|---|---|---|
| Blank | 0 | 10 | 10 | 70 | |
| Pre-Gal | 1/1000 | 10 | 10 | 10 | 60 |
| 1/10,000 | 10 | 10 | 10 | 60 | |
| 1/100,000 | 10 | 10 | 10 | 60 | |
| Auto-Gal | 1/1000 | 10 | 10 | 10 | 60 |
| 1/10,000 | 10 | 10 | 10 | 60 | |
| 1/100,000 | 10 | 10 | 10 | 60 |
