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. Author manuscript; available in PMC: 2012 May 10.
Published in final edited form as: Metabolism. 2008 Oct;57(10):1328–1339. doi: 10.1016/j.metabol.2008.01.036

Figure 3.

Figure 3

Effect of linoleic acid (LA) and α-linolenic acid (ALA) on TNF-α-induced expression of COX-2. Cells were pretreated with 20 μmol/L of LA or ALA for 24 hours and then exposed to 0.5 ng/mL TNF-α for an additional 8 hours. COX-2 protein levels were measured by Western blotting and normalized according to β-actin expression. Experiments were repeated three times, and the blots shown are representative of one of the experiments. The bar graph shows the corresponding densitometric analysis of the blots. Values are means ± SEM. Different letters represent significant difference among treatment groups.