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. 2001 May 15;98(11):6516–6521. doi: 10.1073/pnas.111440998

Figure 1.

Figure 1

Induction of tobacco RdRP activity by SA and TMV infection. (A) Tobacco leaves were treated by floating on 1 mM SA and harvested at indicated times before preparation and assays of RdRP activity. (B) Tobacco leaves were treated with 1 mM SA, 5-chloroSA (5-CSA), acetylSA (ASA), 3-hydroxybenzoic acid (3HBA), or 4-hydroxybenzoic acid (4-HBA) for 48 h before preparation and assays of RdRP activity. (C) Tobacco plants were inoculated on a low leaf with TMV. Leaf tissues were harvested 15 days after inoculation from the upper systemically infected leaves for preparation and assays for RdRP activity. Migration positions of two RNA size markers (generated by in vitro transcription of the TMV genome and a 26-bp DNA fragment cloned in a pBluscript vector) are indicated.