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. 2001 May 15;98(11):6516–6521. doi: 10.1073/pnas.111440998

Figure 2.

Figure 2

Northern blot analysis of the NtRdRP1 gene expression. Total RNA was prepared from tobacco plants and probed with an 860-bp HindIII fragment of the NtRdRP1 cDNA. (A) Expression of NtRdRP1 in tobacco leaves treated with 1 mM SA for indicated times. (B) Expression of NtRdRP1 in tobacco leaves treated for 24 h with 1 mM SA, 5-chloroSA (5-CSA), acetylSA (ASA), 3-hydroxybenzoic acid (3HBA), or 4-hydroxybenzoic acid (4-HBA). (C) Expression of NtRdRP1 in mock- or TMV-inoculated tobacco plants. Chemical treatments and TMV infection were performed as described in Fig. 1. The ethidium bromide stain of rRNA is shown for each lane to allow assessment of equal loading.