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. 2001 May 15;98(11):6516–6521. doi: 10.1073/pnas.111440998

Figure 3.

Figure 3

Suppression of inducible RdRP activity in transgenic tobacco NtRdRP1 antisense plants. (A) RdRP activity from vector-transformed wild-type plants (wt) or transgenic NtRdRP1 antisense lines 14 (L14) and 18 (L18) after treatment with water (−) or 1 mM SA (+) for 48 h. (B) RdRP activity from wild-type plants (wt) or transgenic antisense lines in upper uninoculated leaves 15 days after mock (−) or TMV (+) infection on lower leaves. (C) Transcript levels of NtRdRP1 in wild-type or antisense lines after treatment with water (−) or 1 mM SA (+) for 48 h. (D) Transcript levels of NtRdRP1 in wild-type or antisense lines in upper systemically infected leaves 15 days after mock (−) or TMV (+) infection on lower leaves. Northern blots were hybridized with an antisense-strand RNA probe transcribed from an 860-bp HindIII fragment of the NtRdRP1 cDNA clone. The ethidium bromide stain of rRNA is shown for each lane.