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. 2001 May 15;98(11):6516–6521. doi: 10.1073/pnas.111440998

Figure 6.

Figure 6

PVX symptom development and virus accumulation in transgenic NtRdRP1 antisense plants. (A) The wild-type (Left) or antisense lines 14 (Center) and 18 (Right) were inoculated on one of the lower leaves with PVX. The plants were photographed 15 days after inoculation. (B) Total RNA was isolated 15 days after PVX inoculation from the inoculated leaves and the fifth leaves directly above the inoculated ones of the wild-type (wt) and antisense lines 14 (L14) and 18 (L18) and probed with a DNA fragment corresponding to the coding region for the PVX coat protein. The ethidium bromide stain of rRNA is shown for each lane. Migration positions of size markers are indicated. (C) Total soluble proteins were isolated 15 days after PVX inoculation from the lower inoculated and upper systemically infected leaves of the wild-type (wt) and antisense lines 14 (L14) and 18 (L18) and probed with a PVX-specific polyclonal antibody.