Fig. 3.

In bushy cells, EPSCs grew in steps with 2 patterns. A: EPSCs in a bushy cell that was held at −65 mV grew in 2 steps, the 1st being small and the 2nd being large. B: a plot of the amplitudes of EPSCs as a function of shock strength also shows the steps. K-means cluster analysis indicated that the response grew in 2 steps. The 1st step was smaller (0.4 nA) than the 2nd (1.4 nA). C: in a different bushy cell, EPSCs grew in more steps and reached a larger maximal value. D: a plot of the growth of EPSCs as a function of shock strength reflects the presence of multiple steps. K-means cluster analysis indicates that EPSCs grew in 5 steps in this cell. E: the distribution of amplitudes of steps in EPSCs over the entire population of bushy cells from which such recordings were made (n = 30) shows that the sizes of steps were multimodal. F: there was little or no correlation between the sizes and total number of steps in bushy cells. Sizes of steps were ∼1.5 nA independent of the estimated number of inputs.