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. Author manuscript; available in PMC: 2013 May 2.
Published in final edited form as: J Am Chem Soc. 2012 Apr 23;134(17):7309–7312. doi: 10.1021/ja3022973

Figure 3.

Figure 3

Two-color confocal microscopy images of microtubules in HeLa cells labeled with Pdot-QD705-streptavidin. The blue fluorescence was from nuclear counterstain Hoechst 34580 and the red fluorescence was from Pdot-QD705-streptavidin. (A) Image of nucleus. (B) Image of microtubules. (C) The overlay of panels (A) and (B). (D–F) Images of negative control samples where cells were incubated with Pdot-QD705-streptavidin but in the absence of biotinylated primary antibody. The scale bars are 20 µm. (G–H) Flow cytometry detection of Pdot-Qdot labeled MCF-7 cells. The purple and pink lines show the fluorescence intensity distributions of Pdot-QD705-streptavidin and Pdot-QD800-streptavidin labeled cells, respectively. The black lines represent the results of negative control samples where no primary Biotin anti-human CD326 EpCAM antibody was present.