(A) Phase-contrast images of EMT morphotypic changes in MCF 10A shCTRL and shTwist1 stable cells treated with TNFα.
(B) Western blot of the EMT markers from cells in (A)
(C) Abrogation of TNFα-mediated cancer stem cell population by Twist1 suppression.
(D) Representative photograph of metastatic lung nodules. 4T1-Luc cells with control and two shTwist1 stable clones were injected into BALB/c mice via the tail vein. The mice then received intraperitoneal injection of saline or 10 μg LPS. Seven days later, the mice were sacrificed and the entire lungs were stained with India ink and resected.
(E) Quantification of the lung nodules in Figure 5C. The error bars represent standard deviation for n=5. *indicates P < 0.0002).