Figure 5.
Binding of BeF to CheY. Wild-type CheY in reaction
buffer containing 10 mM NaF was titrated with BeCl3 in the
absence (○) or presence (□) of 1 mM FliM peptide.
The fraction of CheY bound to peptide is given by the relative decrease
in fluorescence intensity
(ΔI/ΔImax). The
dissociation constants are 7.7 ± 0.7 μM in the absence of
peptide and 0.81 ± 0.04 μM in the presence of peptide.