Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Binding of BeF
to CheY. Wild-type CheY in reaction
buffer containing 10 mM NaF was titrated with BeCl3 in the
absence (○) or presence (□) of 1 mM FliM peptide.
The fraction of CheY bound to peptide is given by the relative decrease
in fluorescence intensity
(ΔI/ΔImax). The
dissociation constants are 7.7 ± 0.7 μM in the absence of
peptide and 0.81 ± 0.04 μM in the presence of peptide.
