Table 1.

Phosphorylation rate constants determined in this study

CheY variant	Ligand	kphos/KS (M−1 ⋅ s−1)*	kdephos (s−1)†
Wild type	None	8.0	0.044
	CheA-P2	1.4	0.038
	CheZ peptide	100	0.045
	CheZ205VE peptide	19	n/d
	FliM peptide	230	0.068
87TA	None	0.055	0.0033
	FliM peptide	1.4	0.0082
106YA	None	0.14	0.046
	FliM peptide	4.4	0.038
109KR	None	0.30	0.0097
	FliM peptide	1.8	0.019

n/d, not determined. 

^*Determined using PAM as phosphodonor. 

^† Determined by radiolabeling.