FIG. 4.

Adgb mRNA expression analysis. (A) Quantitative RT-PCR showing Adgb expression in normal mouse tissues. Bars indicate normalized mRNA levels with standard errors of three different mice. (B) Quantitative RT-PCR of Adgb in testes of younger and older mice (d = days, m = months after birth). (C) In situ hybridization of Adgb antisense RNA to mouse testis cryosections (1,2). Dark signal is concentrated towards the lumen of the seminiferous tubules. A hematoxylin/eosin-stained section (3) is shown for comparison. Smooth muscle cells (a), spermatogonia (b), and Sertoli cells (c) are indicated (magnification 40-fold).