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. 2012 Jan 12;119(17):4047–4055. doi: 10.1182/blood-2011-09-377820

Figure 1.

Figure 1

G6pc3−/− macrophages exhibit impaired trafficking in vivo. Macrophages were isolated from 6- to 8-week-old wild-type (+/+) and G6pc3−/− (−/−) littermates. Monoctyes (SSCloGr-1−/lo/+/CD11b+) and macrophages (F4/80+/CD11b+) were analyzed by flow cytometry. (A) BM and blood monocyte counts in wild-type (n = 8) and G6pc3−/− (n = 8) mice in the absence and presence of thioglycollate (TG). (B) BM and blood macrophage counts in wild-type (n = 8) and G6pc3−/− (n = 8) mice in the absence and presence of TG. (C) The total peritoneal macrophage counts in wild-type (n = 8) and G6pc3−/− (n = 8) mice challenged with TG. (D) Flow cytometric analysis of peritoneal macrophages in control and G6pc3−/− mice and Hema 3-stained cytospins of peritoneal macrophages at magnification of ×400. (E) Quantification of MCP-1 and M-CSF mRNA in peritoneal macrophages by real-time RT-PCR. Expression is normalized to β-actin and measured relative to one wild-type mouse arbitrarily defined as 1. (F) The levels of MCP-1 and M-CSF in peritoneal exudates of wild-type (n = 8) and G6pc3−/− (n = 8) mice after intraperitoneal injection of TG. Data represent the mean ± SEM. **P < .005 and *P < .05.