FIG 5 .
Effect of additional RctB sites on origin dynamics in V. cholerae cells by time-lapse microscopy. The figure shows cell growth and segregation of oriI and oriII foci in cells with either the PBAD plasmid (CVC1111) or the PBAD-RctB sites plasmid (CVC1143). The oriI foci are shown in green and oriII foci in red. The cells were grown on an agarose pad by continually feeding them from underneath with fresh medium (MM) using a homemade flow cell. The numbers indicate the time in minutes at which the cells were imaged. In the time interval tested, oriI foci usually duplicate two times and oriII foci once (oriII foci duplicate later in the cell cycle, thwarting capturing of two full replication cycles for chrII because of mCherry bleaching).