TABLE 1 .
Strains and plasmids used in this study
| Bacteria or plasmid | Relevant description | Source or reference |
|---|---|---|
| E. coli | ||
| BR2846 | DH5∆lac = K-12 recA Δ(argF-lac)U169 | 53 |
| CVC1221 | C600 = F− thi∆1 thr-1 supE44 leuB6 lacY1 tonA21 mcrA− mcrB− λ− | NAIST lab stock |
| V. cholerae | ||
| CVC209 | N16961 El tor; Strr | M. Waldor |
| CVC1111 | CVC209/pRN012 (PBAD) | This study |
| CVC1118 | N16961 hapR+ | 40 |
| CVC1140 | CVC1118 + P1parS-Kn (at +135 kb on chrI) | This study |
| CVC1142 | CVC209/pTVC58 (PBAD-rctA) | This study |
| CVC1143 | CVC209/pRN007 (PBAD-RctB sites) | This study |
| CVC1144 | CVC209; Strr/pRN008 (PBAD-parSII) | This study |
| CVC1146 | CVC1140 + pMTparS-Sp (at +40 kb on chrII) | This study |
| CVC1147 | CVC1146/pRN012 (PBAD) | This study |
| CVC1148 | CVC1146/pTVC58 (PBAD-rctA) | This study |
| CVC1149 | CVC1146/pRN007 (PBAD-RctB sites) | This study |
| CVC1150 | CVC1146/pRN008 (PBAD-parSII) | This study |
| Plasmids | ||
| pALA1840 | Source of pMTparS-Sp cassette; Apr Spr | 54 |
| pPS2 | Coordinates 39216 to 41197 of chrII in pDS132; Cmr | 29 |
| pPS4 | Coordinates 133582 to 135584 of chrI cloned at PstI site of pDS132; Cmr | 40 |
| pPS45 | Source of P1parS-Kn cassette; Apr Knr | 40 |
| pPS47 | Source of P1parS-Kn cassette; Cmr Knr | 40 |
| pPS69 | pALA2705 modified to incorporate XhoI and SacII sites + mCherry-mreB PCR XhoI-SacII fragment; Apr | 29 |
| pRN007 | pTVC58 except that rctA is replaced with RctB sites (PBAD-RctB sites) | This study |
| pRN008 | pTVC58 except that rctA is replaced with a parSII site (PBAD-parSII) | This study |
| pRN009 | pPS2 + NsiI fragment from pPS45 containing pMTparS-Sp cassette cloned at the NsiI site; Cmr Spr | This study |
| pRN010 | pPS69 mreB gene between AatII-SacII replaced with pMTparB AatII-SacII PCR fragment | This study |
| pRN011 | pST52 + lacIq-mCherry-pMTparB-gfp-P1parB fragment (NsiI-HindIII) from pRN010 | This study |
| pTVC58 | pMLB1109 + araC-PBAD-rctA from pTVC38 as EcoRI-SmaI PCR fragment (PBAD-rctA); Apr | 20 |