Table 1.

Primers used in this study

Primers for HTR2C amplification
	forward	reverse	template	size (bp)
Exon 1	TTTAGCCCAAGAGAGCGATG	TAGGGTCTGGGCTAGCAATG	genomic	637
Exon 2	TTCTGGCACTAATGAATATCAGC	CATGGTAAGTCAATAACGTCAAATG	genomic	315
Exon 3	CTGCATCCCCATTATGAGC	TGGACCTTTCCTTGCATCTC	genomic	300
Exon 4	AAATGAAAAATCTATCCTCTTTGC	GGCAGATTGAGGCAAATATAGC	genomic	413
Exon 5A	ACTCTGGGGACAGGAGGAAG	CTGCCATGATGACGAGAATG	genomic	400
Exon 5B	ATGGTGGACGCTTCAAATTC	TGCGCACATTCAATTACCTC	genomic	363
Exon 6	GCCCTAGAAAAGGCAAAATG	TAGCCGCTGCAATTCTACTG	genomic	463
Exon 7A	ACCCTTCCGTGTGCTGTAAC	CAAGCCTTCCCACAAAGAAC	genomic	519
Exon 7B	GAAAGCGTCGAAAGTCCTTG	TCAACATTTTTGCATCGAAC	genomic/cDNA	695
HTR2CX1-2	TTTAGCCCAAGAGAGCGATG	TTGAAGGATGGGGATTCTTG	cDNA	653
HTR2CX2-5	GTAGGCCAAGAATCCCCATC	ACCAATAGGCCAATCAGGTG	cDNA	263
HTR2CX4-5	CATGGTGAACCTGAGGAAAG	GCAGAGACAGTGGCATGA	cDNA	335
HTR2CX5-7	CATGCCACTGTCTCTGCTTG	TGTTGTTGACGAACACCTTG	cDNA	299/204
HTR2CNR		TTGCCCAAACAATAGCAATC	cDNA
HTR2CL	TTCTACAGCGTCCATCATGC	CACCTAAAGAAATTGCCCAAAC	qRT-PCR	143
HTR2CS	TGTCATGCCACTGTCTCTGC	ACACCGATCCAGCGAAATAG	qRT-PCR	145

A list of primers used for PCR amplification of HTR2C exons from both genomic DNA, and cDNA prepared from a transcript pool. Primers were selected following comparison of human and porcine reference sequences to define the putative coding regions of the pig genome