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Details of PCR and RFLPs conditions for polymorphisms studied
| Gene | Polymorphism | Primer sequence | PCR Conditions | Enzyme |
|---|---|---|---|---|
| MMP9 | -1562C/T | (F) GCCCGCTCTGGATTATACG (R) CTATCATCTCCTGGCCCCC |
28 cycles: 94°C 30s, 65°C 30s, 72°C 30s | SphI |
| MMP2 | -735C/T | (F) ATAGGGTAAACCTCCCCACATT (R) GGTAAAATGAGGCTGAGACCTG |
30 cycles: 94°C 30s, 62°C 30s, 72°C 30s | HinfI |
| MMP3 | -1171 5A/6A | (F) TTTCAATCAGGACAAGACgaaGTTT* (R) GATTACAGACATGGGTCACA |
30 cycles: 94°C 300s, 53°C 30s, 72°C 30s | XmnI |
* modifíed bases
