Figure 4. Induction of ER-K-rasG12D activation by tamoxifen treatment is important for cell transformation and cell invasiveness in vitro.
A) The foci formation assay were performed in PEK-C MEFs with or without tamoxifen administration. Bar graph indicated the number of foci observed in one well in 6-well plate. B) The PEK-C MEFs were assayed for anchorage-independent cell growth ability in soft agar in the absence or presence of tamoxifen administration. Bar graph indicated the colonies observed in soft agar per well in 6-well plate. C) The PEK-C MEFs were cultured in matrigel in the absence or presence of tamoxifen for indicated days and the cell invasiveness were assay as described in materials and methods.