Table 1. Characteristics of the primers used for SYBR green Q-PCR assays.
| Target gene | Primera | Primer sequence (5′–3′) | Tm (°C)b | % GC | Ampliconlength (bp) | Accession no. |
| Human CD100 | hCD100_F hCD100_R | GAGAAGCAGCATGAGGTGTA ATGACGGATGTGTAGCTGTG | 57,3 57,3 | 50 50 | 266 | NM_006378 NM_001142287 |
| Human CD72 | hCD72_F hCD72_R | CTGAGCAACATGGAGAACAG GCATAAGTCCTAGTGCGTTG | 57,3 57,3 | 50 50 | 323 | NM_001782 |
| Human CD45 | hCD45_F hCD45_R | CTGACATCATCACCTAGCAG TGCTGTAGTCAATCCAGTGG | 57,3 57,3 | 50 50 | 257 | NM_002838 NM_080921 NM_080922 NM_080923 |
| Human SDHA | hSDHA_F hSDHA_R | AGCATCGAAGAGTCATGCAG TCAATCCGCACCTTGTAGTC | 57,3 57,3 | 50 50 | 398 | NM_004168 |
a
The letters F and R at the end of the primer name indicate forward and reverse orientations, respectively.
b
Theoretical melting temperature (Tm) calculated using the MWG Oligo Property Scan (MOPS).