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. 2012 May 11;7(5):e35232. doi: 10.1371/journal.pone.0035232

Table 2. Antibodies used in the study.

Antibody Specificity Ab dilutionfor IH µg of Ab/samplefor IP§ Ab dilutionfor WB Reference
mAb* MCA1269 Human soluble CD100 1∶50 2 µg 1∶1000 AbD Serotec, Oxford, UK
mAb 610670 Human membrane-bound CD100 1∶5 / 1. 500 BD Transduction Laboratories™, Milan, Italy
mAb MCA2501 Human CD72 1∶25 2 µg 1∶1000 AbD Serotec, Oxford, UK
Rabbit pAb ab10558 Human CD45 1∶10 / 1∶500 Abcam, Cambridge, UK
mAb A5316 Human β-actin / / 1∶5000 Sigma-Aldrich, Milan, Italy
mAb M 0814 Human CD68 1∶80 / / DAKO Cytomation, Glostrup, Denmark
*

mAb, monoclonal antibody; pAb, polyclonal antibody; IH, immunohistochemistry; §IP, immunoprecipitation; WB, western blotting.