Table 2. Antibodies used in the study.
Antibody | Specificity | Ab dilutionfor IH‡ | µg of Ab/samplefor IP§ | Ab dilutionfor WB∥ | Reference |
mAb* MCA1269 | Human soluble CD100 | 1∶50 | 2 µg | 1∶1000 | AbD Serotec, Oxford, UK |
mAb 610670 | Human membrane-bound CD100 | 1∶5 | / | 1. 500 | BD Transduction Laboratories™, Milan, Italy |
mAb MCA2501 | Human CD72 | 1∶25 | 2 µg | 1∶1000 | AbD Serotec, Oxford, UK |
Rabbit pAb† ab10558 | Human CD45 | 1∶10 | / | 1∶500 | Abcam, Cambridge, UK |
mAb A5316 | Human β-actin | / | / | 1∶5000 | Sigma-Aldrich, Milan, Italy |
mAb M 0814 | Human CD68 | 1∶80 | / | / | DAKO Cytomation, Glostrup, Denmark |
mAb, monoclonal antibody; †pAb, polyclonal antibody; ‡IH, immunohistochemistry; §IP, immunoprecipitation; ∥WB, western blotting.