Figure 2. mRNA quality is dependent on tissue age.

(A) Average AQUA scores for the UbC positive control by year. Time series testing after simple exponential smoothing demonstrates a statistically significant upward trend with an obvious split at 1993 (p = 0.0003). (B) Average AQUA scores by year for ESR1 are shown only for patients with positive Path ER protein status. Time series testing after simple exponential smoothing demonstrates a statistically significant upward trend (p = 0.0111). (C) Average nuclear AQUA scores by year for are shown ER protein determined with SP1 only for patients with positive Path ER protein status and demonstrate no significant trend over time. Error bars represent standard deviation.