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. 2012 May 11;7(5):e36531. doi: 10.1371/journal.pone.0036531

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Hen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Liver samples from 2-day-old control chicks treated with PBS (A) or FIV-YFP (B) were analyzed by flow cytometry. One representative image from the control PBS and FIV-YFP treated chick groups is shown. Cells in each sample were selected (window P1 in A) and YFP fluorescence was analyzed (B). While no obvious signals were obtained in the control samples, the rate of transduced liver cells in the samples from FIV-YFP treated chicks was 0.46±0.19% (n = 3). Blue and red mark the YFP-positive and negative cells, respectively. The sample with the highest transduction rate (0.8%) is presented.