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. 2012 May 15;23(10):1846–1859. doi: 10.1091/mbc.E11-12-1057

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© 2012 Hoffman et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Uniaxial cyclic stretch induces changes in focal adhesion constituents and actin cytoskeletal reinforcement. Wild-type (WT) fibroblasts on unstretched membranes (ø) or after uniaxial cyclic stretch (2 h, 15%, 0.5 Hz), were fixed and immunostained for zyxin (A, B), Mena/VASP (C, D), and phosphotyrosine (E, F). The direction of stretch is shown in the horizontal plane, designated by a double-headed arrow scaled to 20 μm. Uniaxial cyclic stretch of WT (G, H) and zyxin-null (I, J) fibroblasts followed by phalloidin staining indicated abrogated actin thickening response in the absence of zyxin. (K) SFTI analysis of phalloidin-stained WT (black bars) and zyxin^−/− (white bars) fibroblasts, unstretched (ø) and after 1 h of uniaxial cyclic stretch (+). (L) SFTI analysis of WT fibroblasts stretched in the presence of actinomycin D or cycloheximide (gray bars). ****p < 0.0001, n >100 SFTI measurements.