Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 May 15;23(10):1846–1859. doi: 10.1091/mbc.E11-12-1057

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Hoffman et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 2: — Posttranslational modification of zyxin in response to stretch. (A) Immunoblot analysis of zyxin from unstretched (ø) and stretched (+) WT fibroblasts (lanes 1 and 2) identified a stretch-induced shift of zyxin, which was reversed after incubation with phosphatase (lanes 3 and 4). (B) Immunoblot analysis with a phosphospecific zyxin antibody on unstretched (ø) and stretched (+) cell lysates (lanes 1and 2) and elimination of the phospho-zyxin signal by incubation with phosphatase (lane 3). Immunoblots for total zyxin and β-actin show that their signals are retained following phosphatase treatment. (C, D) Indirect immunofluorescence microscopy on unstretched (ø) and stretched (+) fibroblasts with phospho-zyxin antibody. Phospho-zyxin signal is low at focal adhesions of unstretched cells, but it increases and appears along stress fibers in stretched cells. Stretch direction is in the horizontal plane (double-headed arrow; scale, 20 μm).